In order to isolate, characterize, and establish culture cell lines with different diagnostic and prognostic significance, derived from multiclonal neoplasms, a ductal infiltrating mammary tumor was induced in rats by 7,12-dimethylbenz[a]anthracene. Clones with different DNA/protein content, being the DI of 1.16, 1.30, and 1.60, respectively, were observed in the primary tumor. Biparametric flow cytometry suggested that the clone at 1.30 is made up of two subpopulations with different protein and slightly different DNA contents. The culture, after a few passages, exhibited the presence of aneuploid cells and the absence of diploid components, demonstrating that only tumor cells survived. The limiting dilution method gave rise to four lines with DI of 1.16, 1.25, 1.30, and 1.50; a mean chromosome number of 45, 46, 47, and 88, respectively; and different morphological and ultrastructural features. These characteristics were stable during the experimental procedure, that is, for about 20 passages. Conversely, the detection of cytoskeletal proteins indicated that the tumor epithelial cells underwent early dedifferentiation into sarcoma-like cells showing markers of stromal cell type and thus exhibiting phenotypic instability in vitro, a feature reported in many advanced human breast cancers in vivo. In conclusion, this cellular model represents the in vivo situation and appears suitable for in vitro studies of tumor cell characteristics and might be used to predict clinical behavior.
How to translate text using browser tools
1 March 2000
SELECTION, ESTABLISHMENT AND CHARACTERIZATION OF CELL LINES DERIVED FROM A CHEMICALLY-INDUCED RAT MAMMARY HETEROGENEOUS TUMOR, BY FLOW CYTOMETRY, TRANSMISSION ELECTRON MICROSCOPY, AND IMMUNOHISTOCHEMISTRY
LAURA TEODORI,
FAUSTO TAGLIAFERRI,
FRANCESCO STIPA,
MARIA GIOVANNA VALENTE,
DARIO COLETTI,
ANGELO MANGANELLI,
MARIO GUGLIELMI,
LUCIANA SANTORO D'ANGELO,
HARTMUT SCHÄFER,
WOLFGANG GÖHDE
ACCESS THE FULL ARTICLE
It is not available for individual sale.
This article is only available to subscribers.
It is not available for individual sale.
It is not available for individual sale.
In Vitro Cellular & Developmental Biology - Animal
Vol. 36 • No. 3
March 2000
Vol. 36 • No. 3
March 2000
Breast cancer
DNA content
flow cytometry
rat cell lines
tumor heterogeneity